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Tissue expression of two alpha-amylase genes in the Pacific oyster Crassostrea gigas. Effects of two different food rations ArchiMer
Huvet, Arnaud; Daniel, Jean-yves; Quere, Claudie; Dubois, Solene; Prudence, Marie; Van Wormhoudt, A; Sellos, D; Samain, Jean-francois; Moal, Jeanne.
A semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to estimate tissue-specific expression of two amylase genes in five different tissues of the Pacific oyster Crassostrea gigas and the experimental effects of food level on the cellular, enzymatic and gene expression changes of the digestive gland. Results indicated that both genes were transcribed at different levels depending on tissue type. A strong preferential expression of both amylase genes was observed in the digestive gland leading to high amylase activities, in accordance with the digestive function of this organ. Traces of gene expression and activity were observed in other analysed tissues. These appeared higher in labial palps than in other...
Tipo: Text Palavras-chave: Oyster; Gene expression; Food regulation; Digestive enzyme activity; Alpha amylase.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-394.pdf
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Trophic niche of two co-occurring ophiuroid species in impacted coastal systems, derived from fatty acid and stable isotope analyses ArchiMer
Blanchet-aurigny, Aline; Dubois, Stanislas; Quere, Claudie; Guillou, Monique; Pernet, Fabrice.
The trophic niches of 2 common co-occurring ophiuroids, Ophiocomina nigra and Ophiothrix fragilis (Echinodermata, Ophiuroidea), in 2 contrasting coastal systems of Brittany (France) were investigated. We used a combination of fatty acid biomarkers derived from neutral lipids and stable isotopic compositions to explore the contributions of oceanic versus continental inputs to the ophiuroids' diet. We investigated 2 different systems with an inshore versus offshore comparison. We sampled potential food sources and surveyed organisms every 2 mo for 1 yr. Spatio-temporal variations in stable isotopes and fatty acid profiles of the ophiuroids were generally low compared to interspecific differences. Fatty acid markers showed that both ophiuroids relied on...
Tipo: Text Palavras-chave: Echinoderm; Feeding ecology; Ophiocomina nigra; Ophiothrix fragilis; Trophic markers; Green algae.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00270/38144/36278.pdf
Registros recuperados: 42
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